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gd recombinant human igf i igf 1 protein r d systems  (R&D Systems)


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    R&D Systems gd recombinant human igf i igf 1 protein r d systems
    Gd Recombinant Human Igf I Igf 1 Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gd+recombinant+human+igf+i+igf+1+protein+r+d+systems/Recombinant+Human+GDNF+Protein/pmc11440053__mmc4-156-89-94
    Average 96 stars, based on 280 article reviews
    gd recombinant human igf i igf 1 protein r d systems - by Bioz Stars, 2026-09
    96/100 stars

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    Recombinant:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    SYBR Green Assay:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    Single Cell:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    cDNA Synthesis:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    Quantitative RT-PCR:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    Gene Expression:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS

    Software:

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 Report ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 Report ll OPEN ACCESS

    Article Title: 3D model for human glia conversion into subtype-specific neurons, including dopamine neurons.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALDH1a Abcam Cat# ab24343; RRID: AB_2224007 APC anti-CD44 Miltenyi Biotec Cat# 130-095-177; RRID: AB_10839563 APC anti-human CD133/1 Miltenyi Biotec Cat# 130-113-668; RRID: AB_2726210 DCC Millipore Cat# AB1569; RRID: AB_90789 FITC anti-human SSEA-4 BioLegend Cat# 330410; RRID: AB_1089204 GFAP Covance Cat# SMI-21R-500; RRID: AB_509979 GFAP Millipore Cat# AB5541; RRID: AB_177521 GFP Abcam Cat# ab13970; RRID: AB_300798 GIRK2 Alamone Labs Cat# APC-006; RRID: AB_2040115 HuC/D Thermo Fisher Scientific Cat# A-21271; RRID: AB_221448 INA Thermo Fisher Scientific Cat# PA5-82332; RRID: AB_2789491 LMX1A Santa Cruz Biotechnology Cat# sc-54274; RRID: AB_2297030 Nurr1 Abcam Cat# ab41917; RRID: AB_776887 O4 Millipore Cat# MAB345; RRID: AB_11213138 PCNA Abcam Cat# ab92552; RRID: AB_10561973 PDGFRa Cell Signaling Technology Cat# 5241S; RRID: AB_10692773 PDGFRa R&D Systems Cat# AF-307-NA; RRID: AB_354459 PE anti-human CD140a BD Biosciences Cat# 556002; RRID: AB_396286 SOX10 R&D Systems Cat# AF2864; RRID: AB_442208 STEM123/hGFAP Takara Bio Cat# Y40420; RRID: AB_2833249 TAU (HT7) Thermo Fisher Scientific Cat# MN1000; RRID: AB_2314654 TH Millipore Cat# AB152; RRID: AB_390204 TH Millipore Cat# AB1542; RRID: AB_90755 Chemicals, peptides, and recombinant proteins Accutase Thermo Fisher Scientific Cat# A1110501 Ambion RNase Inhibitor Thermo Fisher Scientific Cat# AM2682 Antibiotic-Antimycotic Thermo Fisher Scientific Cat# 15240096 B27 supplement Thermo Fisher Scientific Cat# 12587010 Biotin Sigma-Aldrich Cat# B4639 BSA Fraction V Thermo Fisher Scientific Cat# 15260037 CHIR99021 Axon Medchem Cat# 1386 DAPI Sigma-Aldrich Cat# D9542 db-cAMP Sigma-Aldrich Cat# D0260 Dichloromethane Thermo Scientific Chemicals Cat# 124050010 DMEM/F12 Thermo Fisher Scientific Cat# 11330-032 DNAse Qiagen Cat# 79256 Donkey serum Biowest Cat# S2170 Dopamine Sigma-Aldrich Cat# H8502 Doxycycline Duchefa Biochemie Cat# D0121 Draq7 BD Biosciences Cat# 564904 EDTA-free protease inhibitor Roche Cat# 04693159001 Ethyl cinnamate Sigma-Aldrich Cat# 112372 FluorSave Reagent Millipore Cat# 345789 (Continued on next page) e1 Cell Reports Methods 4, 100845, September 16, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human recombinant laminin 521 BioLamina Cat# LN521-02 Laminin Thermo Fisher Scientific Cat# 23017015 LDN-193189 Axon Medchem Cat# 1509 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 LM-22A4 R&D Systems Cat# 4607 MEM NEAA Thermo Fisher Scientific Cat# 11140050 N1 Medium Supplement Sigma-Aldrich Cat# N6530 NDiff227 Takara Bio Cat# Y40002 OCT cryomount Histolab Cat# 45830 Paraformaldehyde 4% Sigma-Aldrich Cat# 158127 Phenol red solution Sigma-Aldrich Cat# P0290 Poly-L-Ornithine Sigma-Aldrich Cat# P4957 Propidium iodide, PI Miltenyi Biotec Cat# 130-093-233 Recombinant Human GDNF Protein R&D Systems Cat# 212-GD Recombinant Human IGF-I/IGF-1 Protein R&D Systems Cat# 291-G1 Recombinant Human Noggin Protein Miltenyi Biotec Cat# 130-103-456 Recombinant Human NT-3 Protein R&D Systems Cat# 267-N3 Recombinant Human PDGF-AA Protein R&D Systems Cat# 221-AA SB-431542 Axon Medchem Cat# 1661 StemMACS iPS-Brew XF, human Miltenyi Biotec Cat# 130-104-368 SUPERase$In RNase Inhibitor Thermo Fisher Scientific Cat# AM2696 T3 Sigma-Aldrich Cat# T5516 Tetracycline Sigma-Aldrich Cat# T7660 Triton X-100 Thermo Fisher Scientific Cat# A16046AE UltraPure 0.5M EDTA, pH 8.0 Thermo Fisher Scientific Cat# 15575-038 Valproic Acid (VPA) Millipore Cat# 676380 Critical commercial assays Chromium Next GEM Single Cell 30 GEM, Library & Gel Bead Kit 10x Genomics Cat# PN-1000121 Maxima First Strand cDNA Synthesis Kit for RT-qPCR Thermo Fisher Scientific Cat# K1641 RNeasy Micro Kit Qiagen Cat# 74004 Deposited data Gene Expression Omnbius NCBI GEO: GSE242076 Experimental models: Cell lines GRABDA1H sniffer cells Gift from Freja Herborg and Ulrik Gether Klein Herenbrink et al., 26 Sun et al.27 RC17 hESCs Roslin Cells hPSCreg RCe021-A Oligonucleotides Primers for RT-qPCR (see Table S2) Integrated DNA Technologies N/A Software and algorithms bcl2fastq Bioconductor v2.19 and v2.20 Cell Ranger 10x Genomics V6.0 Clampfit Molecular Devices v10.3 EnhancedVolcano Bioconductor v1.14 FACSDiva software BD Biosciences V9.4 fgsea R Bioconductor v1.22 FlowJo software FlowJo v10.8 GrCH38 10x Genomics v2020-A (Continued on next page) Cell Reports Methods 4, 100845, September 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Harmony Bioconductor v1.2 Igor Pro Wavemetrics v8.04 ImageJ NIH v2.3.0/1.53q LAS X software Leica N/A MSigDB GSEA v7.5.1 NeuroMatic package NeuroMatic v3.0 http://www.neuromatic.thinkrandom. com NIS Elements software Nikon N/A Photoshop 2024 Adobe v25.0.0 Prism 10 GraphPad v10.2.3 R The R Project v4.2.10/4.1 https://www.r-project.org/ Seurat Bioconductor v4.3.1 OPEN ACCESS



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